应用Excel软件分析敌敌畏农药对两广二号家蚕的安全间隔期

应用Excel软件中的曲线方程分析判断敌敌畏农药对两广二号家蚕的安全间隔期。结果表明：敌敌畏1000倍液的安全间隔期是6d多，其数学模型为幂函数曲线方：y=88．0653x-1834本方法克服以往家蚕的农药安全期无数学模型可循或使用的BASIC方法陈旧繁杂的缺点，也避免英文统计软件SPSS难以在农村普及的不足，是一种计...     

Optimum Level of Dietary n‐3 Highly Unsaturated Fatty Acids for Juvenile Sea Cucumber,Apostichopus japonicus

A feeding trial was conducted to estimate the optimum level of dietary n‐3 highly unsaturated fatty acids (HUFAs) for juvenile sea cucumber, Apostichopus japonicas, based on growth performance and fatty acid compositions. Diets with five n‐3 HUFAs levels (0.15, 0.22, 0.33, 0.38, and 0.46%) were fed to sea cucumber juveniles (1.97 ± 0.01 g) once a day for 60 d. The sea cucumbers fed diets containing 0.22% n‐3 HUFAs showed significantly (P < 0.05) higher body weight gain, feed efficiency, and protein efficiency ratio than the sea cucumbers fed diets containing 0.15% n‐3 HUFAs, but not significantly different (P > 0.05) from those of sea cucumbers fed diets containing 0.33, 0.38, and 0.46% n‐3 HUFAs. The sea cucumbers fed diets containing 0.46% n‐3 HUFAs showed significantly (P < 0.05) higher eicosapentaenoic acid and saturated fatty acid than the sea cucumber fed diets containing 0.15% n‐3 HUFAs, but not significantly different (P > 0.05) from those of sea cucumbers fed diets containing 0.22, 0.33, and 0.38% n‐3 HUFAs. The results of growth performance and n‐3 HUFA compositions of body wall indicated that the optimum level of dietary n‐3 HUFAs for juvenile sea cucumber is between 0.22 and 0.46%.     

Interspecific cross‐hybrids of Nicotiana tabacum L. cv. (gla.) S ‘K326’ with Nicotiana alata

In order to introduce the Tomato Spotted Wilt Virus (TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility (CMS) line of N. tabacum (N. tabacum L. cv. (gla.) S ‘K326’), was successfully crossed with N. alata. Despite a high DNA content variability, F₁ hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F₁ plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F₁ subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N. tabacum.     

浅谈我国木薯种收机械化技术

阐述了我国木薯种植、收获机械化技术发展的必要性以及发展现状,同时对我国木薯种收机械化技术发展面临的诸多问题做了详细分析,针对性地提出了解决影响木薯种收机械化技术的对策和几点建议。     

手扶拖拉机专用深松机果园试验

设计了手扶拖拉机配套的专用深松机,并在苹果园进行了田间对比试验.试验结果表明,深松作业降低了苹果园土壤容积密度,改善土壤孔隙状况,土壤贮水库容增加,深松两年后果园土壤0～1 m土层内贮水量较耕翻处理高24.8 mm.深松第3年土壤有机质、全氮含量分别较翻耕及旋耕高0.70 g/kg、0.18 mg/kg及0.43 g/kg、0.16 mg/kg.手扶拖拉机专用深松机作为、一种果园土壤耕作机械在渭北地区有较好的推广前景.     

Effect of ammonia and nitrite on vigour,survival rate,moulting rate of the blue swimming crab <Emphasis Type="Italic">Portunus pelagicus</Emphasis> zoea

The effects of ammonia and nitrite on vigour, survival rate, moulting rate of zoea of blue swimming crab, Portunus pelagicus, were studied. A total of five nitrite-N treatments (26.67, 53.34, 106.68, 213.36, 426.72 mg/l) and a control (no nitrite-N added) were set up for the acute nitrite-N toxicity experiment; a total of five ammonia-N treatments (8.43, 16.86, 33.72, 67.44, 134.88 mg/l) and a control (no ammonia-N added) were set up for the acute ammonia-N toxicity experiment. The results showed that the vigour, survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 53.34 mg/l were significantly different (P < 0.05) from the control group. The zoea LC₅₀ values (mg/l) of nitrite-N were 179.47, 76.56, 66.70, 37.49, 25.01, 25.35, 25.34 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively. The vigour, survival rate and moulting rate of zoea of the blue swimming crabs exposed to over 16.86 mg/l were significantly different (P < 0.05) from the control group. The zoea LC₅₀ values (mg/l) of ammonia-N were 51.04, 39.62, 38.72, 24.43, 16.90, 13.42, 11.16 mg/l for 24, 36, 48, 60, 72, 84, 96 h, respectively. The zoeae are highly sensitive to ammonia and nitrite, and the toxicity of ammonia and nitrite on Portunus pelagicus decrease with development of this crab.     

农业机械化领域农业推广硕士培养质量保障调查研究

以农业机械化领域农业推广硕士为研究对象,采取纸质问卷调查、网络调查、座谈会和个别访谈等相结合的方法对其培养质量保障问题进行了实证研究。从人才培养单位、人才使用单位、人事部门和学员自身4个方面分析了影响推广硕士培养质量的因素,从而得到推广硕士培养质量保障问题的一些结论,以期促进推广硕士培养质量的提高。     

基于小波支持向量机的机动管道排空时间预测

机动管道在输送油品和地形条件固定的情况下,管道长度、空压机的排气压力和排气量是影响管道排空作业时间的主要因素,但几种因素对排空时间的影响呈现复杂的非线性关系.以机动管道排空过程的实验数据为基础,分析了排空时间随各影响因素的变化规律.通过分析,发现各因素与排空时间之间具有较好的关联性,用支持向量回归的方法可以对排空时间进行预测.在对样本数据进行训练和预测时,采用基于morlet小波核的支持向量回归方法,并与高斯核的预测效果进行对比,发现小波核比高斯核具有更好的排空时间预测效果,并给出了管道气顶排空时间预报公式.     

鹦哥岭自然保护区海南锯腿树蛙种群分布以及繁殖生物学研究

2011年2月~2012年7月,对海南鹦哥岭自然保护区内的海南锯腿树蛙(Rhacophorus hainanus)种群分布进行了调查,用野外观察与室内饲养的方法对其繁殖生物学进行了初步研究。结果表明:海南锯腿树蛙分布在鹦哥岭自然保护区内海拔800m以上的溪流边或是季节性水潭附近;该蛙具有明显的性二型特点;繁殖季节为每年的3月至6月,繁殖地为季节性水潭,繁殖包括鸣叫求偶、抱对、产卵三个过程,每次产卵量为126±77枚,蛙卵约7d孵化为蝌蚪,57d蝌蚪变态成为幼蛙。该蛙唇齿式为I:4+4/1+1:II,与云南部分蝌蚪标本以及越南采集的蝌蚪标本有差异。     

Molecular identification and relative abundance of microorganisms in douchi koji and salted egg white sufu during processing

A novel animal protein-based douchi koji-inoculated steamed salted egg white sufu (SEWS) has been developed. This study determined the relative abundance of microorganisms in the douchi koji and semi-finished (5-day fermentation) and finished (5-day fermentation and 14-day ripening) SEWS by using 16S and 18S ribosomal DNA and gene-cloning methods. The results revealed that Bacillus spp. and Aspergillus oryzae were dominant in the douchi koji. In the semi-finished SEWS, the percentages of Bacillus amyloliquefaciens and Bacillus subtilis were considerably lower, whereas those of Enterococcus and Staphylococcus were substantially higher. In the finished SEWS, Bacillus spp. became dominant again and A. oryzae was the only fungus detected. In conclusion, by using molecular techniques, microbial population dynamics in SEWS can be evaluated. During processing, the relative abundance of microorganisms in SEWS changed and Bacillus spp. and A. oryzae remained dominant. This study provides crucial information for designing starter cultures for producing SEWS.